Generator
Part:BBa_K2055025:Design
Designed by: Martha Carolina Elizondo Cantú Group: iGEM16_Tec-Monterrey (2016-09-29)
Sulfur reductase generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It should be used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase (DE3 systems).
Source
The sequence of the sulfur reductase was obtained from UniProt. It corresponds to the SOR gene of Acidianus ambivalens, accession number P29082.
The UniProt Consortium. UniProt: a hub for protein information. Nucleic Acids Res. 43: D204-D212 (2015)